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Frequency: Quarterly
ISSN: 1553-3468 (Print)
ISSN: 1558-6332 (Online)
Research Article Open Access

Preparation, Characterization and Antioxidant Activity of a Novel Acetylated Polysaccharide from Flammulina Velutipes

Yingyun Peng1, Han Jiang1, Yufeng Wu1, Jinlong Zhang1, Jianhua Zhou2 and Yiyong Chen1
  • 1 School of Biology and Food Engineering, Changshu Institute of Technology, China
  • 2 Center of Technology and Development, Zhangjiagang Tianyuan Ecological Breeding Co., Ltd, China

Abstract

In this study, a novel polysaccharide FVP2a wasextracted and purified from F. velutipes. Ac-FVP2a was prepared bychemical acetylation. The preparation process of Ac-FVP2a was optimized usingthe response surface method based on three experimental factors includingreaction time, reaction temperature, and the ratio of FVP2a to aceticanhydride. The physicochemical characteristics andantioxidant activities of Ac-FVP2a in vitro were investigated. The results showed that the optimalpreparation process of Ac-FVP2a was a reaction time of 2.5 h, reactiontemperature of 45℃ and ratio of FVP2ato acetic anhydride of 1:37 (g/mL), respectively. The monosaccharides of Ac-FVP2awith an average molecular weight of 1.97 × 105 Da were galactose,glucose, rhamnose, xylose, and arabinose and the molar ratio were1.65:1:0.13:0.26:1.12. FT-IR suggested that Ac-FVP2a was prepared successfully.Ac-FVP2a had stronger antioxidant activities with better thermal stability thanFVP2a. Ac-FVP2a might have the potential to become a novel antioxidant in thefood industry.

American Journal of Biochemistry and Biotechnology
Volume 18 No. 3, 2022, 339-348

DOI: https://doi.org/10.3844/ajbbsp.2022.339.348

Submitted On: 10 February 2022 Published On: 20 September 2022

How to Cite: Peng, Y., Jiang, H., Wu, Y., Zhang, J., Zhou, J. & Chen, Y. (2022). Preparation, Characterization and Antioxidant Activity of a Novel Acetylated Polysaccharide from Flammulina Velutipes. American Journal of Biochemistry and Biotechnology, 18(3), 339-348. https://doi.org/10.3844/ajbbsp.2022.339.348

Open AccessCopyright: © 2022 Yingyun Peng, Han Jiang, Yufeng Wu, Jinlong Zhang, Jianhua Zhou and Yiyong Chen. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Keywords

  • Polysaccharide
  • Flammulina Velutipes
  • Acetylation
  • Antioxidant Activity