@article {10.3844/ajbbsp.2026.22.03.043,
article_type = {journal},
title = {Effects of Aerobic Exercise on PPAR-γ Expression in Adipose Tissue and RT-PCR Detection Analysis},
author = {Zhan, Xiaodong and Bi, Chenguang and Wei, Jie},
volume = {22},
number = {3},
year = {2026},
month = {Sep},
pages = {43-1},
doi = {10.3844/ajbbsp.2026.22.03.043},
url = {https://thescipub.com/abstract/ajbbsp.2026.22.03.043},
abstract = {This study aimed to examine the effect of organized aerobic exercise on the expression level of Peroxisome Proliferator-Activated Receptor gamma (PPAR-γ) gene in adipose tissue by performing digital variant of RT-PCR, Droplet Digital PCR (ddPCR) which is a highly sensitive method for counting the gene copy numbers. The proposed scheme consists of three-stage analytical procedure, namely, Subphase I Controlled Aerobic Intervention Modeling, which optimizes exercise intensity by normalizing physiological parameters; Subphase II High-Precision Molecular Quantification, which applies ddPCR with Poisson-based absolute copy number estimation to estimate gene-expression profiles; and Subphase III Algorithmic Statistical Inference, which constructs robust gene-expression profiles using normalization algorithms and variance stabilization modeling, as well as multivariate effect analysis. The results indicated that the aerobic group had 28–35% higher expression of PPAR-γ mRNA compared to the control group (p},
journal = {American Journal of Biochemistry and Biotechnology},
publisher = {Science Publications}
}