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Frequency: Quarterly
ISSN: 1608-4217 (Print)
ISSN: 2410-8561 (Online)
Research Article Open Access

Assessment of Genetic Purity of Parental Lines of Hybrid Rice Using DNA-Based Markers

Sunil Bhavsar1, Twinkle Solanki1, Suchita Amin1 and Neeru Jain2
  • 1 Veer Narmad South Gujarat University, India
  • 2 Barwale knowledge and Study Centre, India

Abstract

Assessment of hybrid seed genetic purity is one of the most important quality control parameters in hybrid seed production. With the objective of replacing the GOT with DNA based assays, Cytoplasmic Male Sterile (CMS), restorer and hybrid lines have been screened by means of microsatellite and Sequence Tagged Site (STS) markers. Cytoplasmic Male Sterile (CMS) lines often get contaminated with cognate isonuclear maintainer lines during multiplication. The PCR assay was able to detect precisely the impurities in a commercial seed lot of CMS line. The extent of heterozygosity within parental lines of rice hybrids were assessed and the results suggest that a single, appropriately chosen microsatellite marker should be sufficient for assessing hybrid seed purity.

OnLine Journal of Biological Sciences
Volume 15 No. 2, 2015, 59-69

DOI: https://doi.org/10.3844/ojbsci.2015.59.69

Submitted On: 22 March 2015 Published On: 13 June 2015

How to Cite: Bhavsar, S., Solanki, T., Amin, S. & Jain, N. (2015). Assessment of Genetic Purity of Parental Lines of Hybrid Rice Using DNA-Based Markers. OnLine Journal of Biological Sciences, 15(2), 59-69. https://doi.org/10.3844/ojbsci.2015.59.69

Open AccessCopyright: © 2015 Sunil Bhavsar, Twinkle Solanki, Suchita Amin and Neeru Jain. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Keywords

  • Hybrid Seed
  • Cytoplasmic Male Sterile
  • PCR
  • Heterozygosity