Research Article Open Access

Using Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction of Barbodes spp. in Thailand

Keeravit Petjul1, Aphidech Sangdee2 and Bungorn Thaewnon-ngiw2
  • 1 Rajamangala University of Technology Isan, Thailand
  • 2 Mahasarakam University, Thailand

Abstract

Problem statement: The genetic diversity of Barbodes species in Thailand is presently unavailable. The objectives of this study were to study external morphology and genetic diversity using Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) of three Barbodes species; Barbodes altus, B. gonionotus and Barbodes sp. Approach: Two of twelve screened decanucleotide primers (OPA07 and UBC122), were selected for the genetic analysis of Barbodes genera. Results: Thirty-five reproducible and polymorphic fragments (250-2150 bp in length) were generated across the investigated species. The percentages of polymorphic band were 7.69, 3.85 and 6.25% for B. altus, B. gonionotus and Barbodes sp., respectively. The UPGMA indicated a similarity coefficient between B. gonionotus and Barbodes sp. up to 90%. The candidate species-specific marker of UBC122 primer was found in only Barbodes sp. Conclusion: This approach indicates that Barbodes sp. may be a variant species of B. gonionotus.

OnLine Journal of Biological Sciences
Volume 11 No. 1, 2011, 1-6

DOI: https://doi.org/10.3844/ojbsci.2011.1.6

Submitted On: 17 June 2010 Published On: 6 July 2011

How to Cite: Petjul, K., Sangdee, A. & Thaewnon-ngiw, B. (2011). Using Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction of Barbodes spp. in Thailand. OnLine Journal of Biological Sciences, 11(1), 1-6. https://doi.org/10.3844/ojbsci.2011.1.6

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Keywords

  • Genetic diversity
  • Barbodes
  • DNA analysis
  • RAPD-PCR