Studies on Emergence of Drug Resistance in HIV Associated Bacterial Urinary Tract Infections

Abstract

Problem statement: This study was designed to document the prevalence of HIV associated urinary tract infections in Barshi Tahsil. Such studies had not been researched out before in this region. Approach: The study included samples from 123 HIV reactive and 40 HIV non-reactive patients. In both, mid stream samples were collected and processed to examine for bacterial opportunistic pathogens. The antibiogram of selected urinary isolates in HIV reactive as well as HIV non-reactive patients was studied by using disc diffusion technique and Kirby Bauer method. Study regarding molecular approach is in progress. Results: Urine samples from 75.46% of HIV reactive and 24.54% of HIV non reactive patients were culture positive. In all there were 74 urinary isolates from the HIV reactive cases. About (36.48%) were E. coli, (31.10%) were Pseudomonas aeruginosa and (24%) were Klebsiella. These urinary isolates were found in different age groups of both male and female but more number of isolates was found in female as compared to male. In addition, E. coli and Klebsiella were predominant in female and Pseudomonas aeruginosa was found predominant in male. The antibiogram of selected urinary isolates in HIV reactive as well as HIV non-reactive patients indicates that urinary isolates in HIV reactive patients were more drug resistant than HIV non-reactive patients. Conclusion: The isolation of urinary opportunistic pathogens among the HIV reactive patients was found to be significantly higher than in HIV nonreactive patients. Also the antibiogram of urinary opportunistic pathogens in HIV reactive patients was significant finding indicating severity of the infection in this group.